PrimePCR™ SYBR® Green Assay: DCAF12, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0026915

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: DCAF12, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DCAF12, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0008696
Assay Design:   Intron-spanning
Chromosome Location:   9:34107499-34125078question
Amplicon Length:   93
Splice Variants Targeted:   ENST00000361264 ENST00000396990 ENST00000450964

Gene Information

This gene encodes a WD repeat-containing protein that interacts with the COP9 signalosome a macromolecular complex that interacts with cullin-RING E3 ligases and regulates their activity by hydrolyzing cullin-Nedd8 conjugates. [provided by RefSeq Jul 2009]

Gene Symbol:   DCAF12
Gene Name:   DDB1 and CUL4 associated factor 12
Aliases:   CT102, DKFZp434O125, KIAA1892, MGC1058, TCC52, WDR40A
RefSeq:   NC_000009.11 NT_008413.18
Ensembl:   ENSG00000198876
Entrez:   25853
Chromosome Mapping:   9p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.020000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download