PrimePCR™ SYBR® Green Assay: GNS, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0026969

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: GNS, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: GNS, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0008836
Assay Design:   Intron-spanning
Chromosome Location:   12:65134412-65137016question
Amplicon Length:   123
Splice Variants Targeted:   ENST00000418919 ENST00000258145 ENST00000543646 ENST00000542058 ENST00000540196 ENST00000545471 ENST00000545273 ENST00000539825

Gene Information

The product of this gene is a lysosomal enzyme found in all cells. It is involved in the catabolism of heparin heparan sulphate and keratan sulphate. Deficiency of this enzyme results in the accumulation of undegraded substrate and the lysosomal storage disorder mucopolysaccharidosis type IIID (Sanfilippo D syndrome). Mucopolysaccharidosis type IIID is the least common of the four subtypes of Sanfilippo syndrome. [provided by RefSeq Jul 2008]

Gene Symbol:   GNS
Gene Name:   glucosamine (N-acetyl)-6-sulfatase
Aliases:   G6S, MGC21274
RefSeq:   NC_000012.11 NG_008955.1 NT_029419.12
Ensembl:   ENSG00000135677
Entrez:   2799
Chromosome Mapping:   12q14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.060000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download