PrimePCR™ SYBR® Green Assay: ST8SIA6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best coverage


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PrimePCR™ PreAmp for SYBR® Green Assay: ST8SIA6, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: ST8SIA6, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0008861
Assay Design:		Intron-spanning
Chromosome Location:		10:17365064-17369092question
Amplicon Length:		143
Splice Variants Targeted:		ENST00000440449 ENST00000377602 ENST00000377610

Gene Information

Sialic acid is a key determinate of oligosaccharide structures involved in cell-cell communication cell-substrate interaction adhesion and protein targeting. ST8SIA6 belongs to a family of sialyltransferases (EC 2.4.99.8) that synthesize sialylglycoconjugates (Takashima et al. 2002 [PubMed 11980897]).[supplied by OMIM Mar 2008]

Gene Symbol:		ST8SIA6
Gene Name:		ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 6
Aliases:		SIA8F, SIAT8F, ST8SIA-VI
RefSeq:		NC_000010.10 NT_008705.16
Ensembl:		ENSG00000148488
Entrez:		338596
Chromosome Mapping:		10p12.33

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999600
y-intercept	34.960000
Efficiency	102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.