PrimePCR™ SYBR® Green Assay: ARID2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0026985

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ARID2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ARID2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0008906
Assay Design:   Intron-spanning
Chromosome Location:   12:46285831-46287320question
Amplicon Length:   137
Splice Variants Targeted:   ENST00000334344 ENST00000549153 ENST00000422737 ENST00000444670 ENST00000457135 ENST00000338636

Gene Information

ARID2 is a subunit of the PBAF chromatin-remodeling complex (see BAF180; MIM 606083) which facilitates ligand-dependent transcriptional activation by nuclear receptors (Yan et al. 2005 [PubMed 15985610]).[supplied by OMIM Mar 2008]

Gene Symbol:   ARID2
Gene Name:   AT rich interactive domain 2 (ARID, RFX-like)
Aliases:   BAF200, DKFZp686G052, DKFZp779P0222, FLJ30619, KIAA1557, p200
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000189079
Entrez:   196528
Chromosome Mapping:   12q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.750000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download