PrimePCR™ SYBR® Green Assay: ABCB4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: ABCB4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ABCB4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0008944
Assay Design:   Intron-spanning
Chromosome Location:   7:87053327-87056114question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000359206 ENST00000265723 ENST00000453593 ENST00000358400 ENST00000545634

Gene Information

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1 MDR/TAP MRP ALD OABP GCN20 White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This gene encodes a full transporter and member of the p-glycoprotein family of membrane proteins with phosphatidylcholine as its substrate. The function of this protein has not yet been determined; however it may involve transport of phospholipids from liver hepatocytes into bile. Alternative splicing of this gene results in several products of undetermined function. [provided by RefSeq Jul 2008]

Gene Symbol:   ABCB4
Gene Name:   ATP-binding cassette, sub-family B (MDR/TAP), member 4
Aliases:   ABC21, GBD1, MDR2, MDR2/3, MDR3, PFIC-3, PGY3
RefSeq:   NC_000007.13 NT_007933.15 NG_007118.1
Ensembl:   ENSG00000005471
Entrez:   5244
Chromosome Mapping:   7q21.1

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