PrimePCR™ SYBR® Green Assay: INPP5A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027011

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: INPP5A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: INPP5A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0008986
Assay Design:   Intron-spanning
Chromosome Location:   10:134421451-134463968question
Amplicon Length:   128
Splice Variants Targeted:   ENST00000368594 ENST00000368593 ENST00000451873 ENST00000342652 ENST00000416326 ENST00000368595 ENST00000432898

Gene Information

The protein encoded by this gene is a membrane-associated type I inositol 145-trisphosphate (InsP3) 5-phosphatase. InsP3 5-phosphatases hydrolyze Ins(145)P3 which mobilizes intracellular calcium and acts as a second messenger mediating cell responses to various stimulation. [provided by RefSeq Jul 2008]

Gene Symbol:   INPP5A
Gene Name:   inositol polyphosphate-5-phosphatase, 40kDa
Aliases:   5PTASE, DKFZp434A1721, MGC116947, MGC116949
RefSeq:   NC_000010.10 NT_008818.16
Ensembl:   ENSG00000068383
Entrez:   3632
Chromosome Mapping:   10q26.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.360000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download