PrimePCR™ SYBR® Green Assay: SLC22A8, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027105

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SLC22A8, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SLC22A8, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009241
Assay Design:   Intron-spanning
Chromosome Location:   11:62763502-62766429question
Amplicon Length:   130
Splice Variants Targeted:   ENST00000336232 ENST00000545207 ENST00000535878 ENST00000311438 ENST00000430500 ENST00000540631

Gene Information

This gene encodes a protein involved in the sodium-independent transport and excretion of organic anions some of which are potentially toxic. The encoded protein is an integral membrane protein and appears to be localized to the basolateral membrane of the kidney. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq May 2010]

Gene Symbol:   SLC22A8
Gene Name:   solute carrier family 22 (organic anion transporter), member 8
Aliases:   MGC24086, OAT3
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000149452
Entrez:   9376
Chromosome Mapping:   11q11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 34.280000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download