PrimePCR™ SYBR® Green Assay: CNOT4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027155

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CNOT4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CNOT4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009343
Assay Design:   Intron-spanning
Chromosome Location:   7:135099155-135106959question
Amplicon Length:   139
Splice Variants Targeted:   ENST00000451834 ENST00000423368 ENST00000361528 ENST00000414802 ENST00000428680 ENST00000262563 ENST00000541284 ENST00000356162 ENST00000315544

Gene Information

The protein encoded by this gene is a subunit of the CCR4-NOT complex a global transcriptional regulator. The encoded protein interacts with CNOT1 and has E3 ubiquitin ligase activity. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq Jul 2010]

Gene Symbol:   CNOT4
Gene Name:   CCR4-NOT transcription complex, subunit 4
Aliases:   CLONE243, NOT4, NOT4H
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000080802
Entrez:   4850
Chromosome Mapping:   7q22-qter

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.150000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download