PrimePCR™ SYBR® Green Assay: CDC42BPA, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


List Price:		$179.00
Your Price:		Log In
Quantity:		Add to Cart Add to Cart
Add to My PrimePCR Add to My PrimePCR		Add to Quote Add to Quote
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: CDC42BPA, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

List Price:		$174.00
Your Price:		Log In
Quantity:		Add to Cart Add to Cart
[ Add to My PrimePCR ] [ Add to My PrimePCR ]		[ Add to Quote ] [ Add to Quote ]

PrimePCR™ Template for SYBR® Green Assay: CDC42BPA, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

List Price:		$179.00
Your Price:		Log In
Quantity:		Add to Cart Add to Cart
[ Add to My PrimePCR ] [ Add to My PrimePCR ]		[ Add to Quote ] [ Add to Quote ]

Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0009361
Assay Design:		Intron-spanning
Chromosome Location:		1:227262010-227268709question
Amplicon Length:		142
Splice Variants Targeted:		ENST00000366769 ENST00000366767 ENST00000442054 ENST00000366765 ENST00000334218 ENST00000366766 ENST00000366764 ENST00000535525 ENST00000366762

Gene Information

The protein encoded by this gene is a member of the Serine/Threonine protein kinase family. This kinase contains multiple functional domains. Its kinase domain is highly similar to that of the myotonic dystrophy protein kinase (DMPK). This kinase also contains a Rac interactive binding (CRIB) domain and has been shown to bind CDC42. It may function as a CDC42 downstream effector mediating CDC42 induced peripheral actin formation and promoting cytoskeletal reorganization. Multiple alternatively spliced transcript variants have been described and the full-length nature of two of them has been reported. [provided by RefSeq Jul 2008]

Gene Symbol:		CDC42BPA
Gene Name:		CDC42 binding protein kinase alpha (DMPK-like)
Aliases:		DKFZp686L1738, DKFZp686P1738, FLJ23347, KIAA0451, MRCK, MRCKA, PK428
RefSeq:		NC_000001.10 NT_167186.1
Ensembl:		ENSG00000143776
Entrez:		8476
Chromosome Mapping:		1q42.11

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.997800
y-intercept	34.110000
Efficiency	105

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.