PrimePCR™ SYBR® Green Assay: FCER1G, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: FCER1G, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: FCER1G, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009583
Assay Design:   Intron-spanning
Chromosome Location:   1:161185092-161187846question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000367992 ENST00000289902

Gene Information

The high affinity IgE receptor is a key molecule involved in allergic reactions. It is a tetramer composed of 1 alpha 1 beta and 2 gamma chains. The gamma chains are also subunits of other Fc receptors. [provided by RefSeq Jul 2008]

Gene Symbol:   FCER1G
Gene Name:   Fc fragment of IgE, high affinity I, receptor for; gamma polypeptide
Aliases:   FCRG
RefSeq:   NC_000001.10 NG_013352.1 NT_004487.19
Ensembl:   ENSG00000158869
Entrez:   2207
Chromosome Mapping:   1q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.060000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download