PrimePCR™ SYBR® Green Assay: SCN1A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027254

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SCN1A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SCN1A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009596
Assay Design:   Intron-spanning
Chromosome Location:   2:166870357-166892564question
Amplicon Length:   149
Splice Variants Targeted:   ENST00000303395 ENST00000375405 ENST00000409050 ENST00000423058

Gene Information

The vertebrate sodium channel is a voltage-gated ion channel essential for the generation and propagation of action potentials mainly in nerve and muscle. Voltage-sensitive sodium channels are heteromeric complexes consisting of a large central pore-forming glycosylated alpha subunit and two smaller auxiliary beta subunits. This gene encodes the large alpha subunit and mutations in this gene have been associated with several epilepsy convulsion and migraine disorders. Alternative splicing results in multiple transcript variants. The RefSeq Project has decided to create four representative RefSeq records. Three of the transcript variants are supported by experimental evidence and the fourth contains alternate 5' untranslated exons the exact combination of which have not been experimentally confirmed for the full-length transcript.[provided by RefSeq Jan 2011]

Gene Symbol:   SCN1A
Gene Name:   sodium channel, voltage-gated, type I, alpha subunit
Aliases:   EIEE6, FEB3, FEB3A, FHM3, GEFSP2, HBSCI, NAC1, Nav1.1, SCN1, SMEI
RefSeq:   NC_000002.11 NT_005403.17 NG_011906.1
Ensembl:   ENSG00000144285
Entrez:   6323
Chromosome Mapping:   2q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.640000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download