PrimePCR™ SYBR® Green Assay: ZP3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027441

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ZP3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZP3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010073
Assay Design:   Intron-spanning
Chromosome Location:   7:76058883-76062328question
Amplicon Length:   123
Splice Variants Targeted:   ENST00000336517 ENST00000394857 ENST00000416245 ENST00000544121

Gene Information

The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in primary binding and induction of the sperm acrosome reaction. The nascent protein contains a N-terminal signal peptide sequence a conserved ZP domain a C-terminal consensus furin cleavage site and a transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However the requirement for furin cleavage in this process remains controversial based on mouse studies. A variation in the last exon of this gene has previously served as the basis for an additional ZP3 locus; however sequence and literature review reveals that there is only one full-length ZP3 locus in the human genome. Another locus encoding a bipartite transcript designated POMZP3 contains a duplication of the last four exons of ZP3 including the above described variation and maps closely to this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   ZP3
Gene Name:   zona pellucida glycoprotein 3 (sperm receptor)
Aliases:   ZP3A, ZP3B, ZPC, Zp-3
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000188372
Entrez:   7784
Chromosome Mapping:   7q11.23

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