PrimePCR™ SYBR® Green Assay: PHF17, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0027540


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PrimePCR™ PreAmp for SYBR® Green Assay: PHF17, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: PHF17, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0010327
Assay Design:		Intron-spanning
Chromosome Location:		4:129752940-129767581question
Amplicon Length:		134
Splice Variants Targeted:		ENST00000511647 ENST00000452328 ENST00000504089 ENST00000512960 ENST00000503785 ENST00000510308 ENST00000413543 ENST00000507833 ENST00000508997 ENST00000535321 ENST00000226319

Gene Information

Description Not Available

Gene Symbol:		PHF17
Gene Name:		PHD finger protein 17
Aliases:		FLJ22479, JADE1, KIAA1807
RefSeq:		NC_000004.11 NT_016354.19
Ensembl:		ENSG00000077684
Entrez:		79960
Chromosome Mapping:		4q26-q27

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999100
y-intercept	35.600000
Efficiency	95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.