PrimePCR™ SYBR® Green Assay: DNAJB12, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0027559


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PrimePCR™ PreAmp for SYBR® Green Assay: DNAJB12, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: DNAJB12, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0010379
Assay Design:		Intron-spanning
Chromosome Location:		10:74096403-74098038question
Amplicon Length:		101
Splice Variants Targeted:		ENST00000444643 ENST00000338820 ENST00000394903

Gene Information

DNAJB12 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain (Ohtsuka and Hata 2000 [PubMed 11147971]).[supplied by OMIM Mar 2008]

Gene Symbol:		DNAJB12
Gene Name:		DnaJ (Hsp40) homolog, subfamily B, member 12
Aliases:		DJ10, DKFZp586B2023
RefSeq:		NC_000010.10 NT_030059.13
Ensembl:		ENSG00000148719
Entrez:		54788
Chromosome Mapping:		10q22.1

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998900
y-intercept	34.920000
Efficiency	104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.