PrimePCR™ SYBR® Green Assay: MAPKAP1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0027562


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PrimePCR™ PreAmp for SYBR® Green Assay: MAPKAP1, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: MAPKAP1, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0010385
Assay Design:		Intron-spanning
Chromosome Location:		9:128322052-128347905question
Amplicon Length:		79
Splice Variants Targeted:		ENST00000373511 ENST00000350766 ENST00000373503 ENST00000373498 ENST00000420643 ENST00000394060 ENST00000427078 ENST00000265960 ENST00000394063 ENST00000373505

Gene Information

This gene encodes a protein that is highly similar to the yeast SIN1 protein a stress-activated protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been described. Alternate polyadenylation sites as well as alternate 3' UTRs have been identified for transcripts of this gene. [provided by RefSeq Jul 2008]

Gene Symbol:		MAPKAP1
Gene Name:		mitogen-activated protein kinase associated protein 1
Aliases:		JC310, MGC2745, MIP1, SIN1, SIN1b, SIN1g
RefSeq:		NC_000009.11 NT_008470.19
Ensembl:		ENSG00000119487
Entrez:		79109
Chromosome Mapping:		9q33.3

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998000
y-intercept	33.800000
Efficiency	105

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.