PrimePCR™ SYBR® Green Assay: SLC2A8, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027626

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SLC2A8, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SLC2A8, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010537
Assay Design:   Intron-spanning
Chromosome Location:   9:130162228-130164942question
Amplicon Length:   135
Splice Variants Targeted:   ENST00000373371 ENST00000451404 ENST00000419917 ENST00000373360 ENST00000419132 ENST00000439597 ENST00000423934 ENST00000373350

Gene Information

Description Not Available

Gene Symbol:   SLC2A8
Gene Name:   solute carrier family 2 (facilitated glucose transporter), member 8
Aliases:   GLUT8, GLUTX1
RefSeq:   NC_000009.11 NT_008470.19
Ensembl:   ENSG00000136856
Entrez:   29988
Chromosome Mapping:   9q33.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.240000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download