PrimePCR™ SYBR® Green Assay: ARAP1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027636

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ARAP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ARAP1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010562
Assay Design:   Intron-spanning
Chromosome Location:   11:72412715-72414007question
Amplicon Length:   142
Splice Variants Targeted:   ENST00000359373 ENST00000393605 ENST00000334211 ENST00000393609 ENST00000426523 ENST00000429686 ENST00000427971 ENST00000452383 ENST00000455638 ENST00000340247

Gene Information

The protein encoded by this gene contains SAM ARF-GAP RHO-GAP ankyrin repeat RAS-associating and pleckstrin homology (PH) domains. In vitro this protein displays RHO-GAP and phosphatidylinositol (345) trisphosphate (PIP3)-dependent ARF-GAP activity. The encoded protein associates with the Golgi and the ARF-GAP activity mediates changes in the Golgi and the formation of filopodia. It is thought to regulate the cell-specific trafficking of a receptor protein involved in apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Sep 2008]

Gene Symbol:   ARAP1
Gene Name:   ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1
Aliases:   CENTD2, KIAA0782
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000186635
Entrez:   116985
Chromosome Mapping:   11q13.4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.380000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download