PrimePCR™ SYBR® Green Assay: PRMT2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027654

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PRMT2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PRMT2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010601
Assay Design:   Intron-spanning
Chromosome Location:   21:48068503-48069598question
Amplicon Length:   111
Splice Variants Targeted:   ENST00000355680 ENST00000397638 ENST00000397637 ENST00000334494 ENST00000397628 ENST00000440086 ENST00000455177 ENST00000458387 ENST00000451211 ENST00000291705 ENST00000379844

Gene Information

Description Not Available

Gene Symbol:   PRMT2
Gene Name:   protein arginine methyltransferase 2
Aliases:   HRMT1L1, MGC111373
RefSeq:   NC_000021.8 NT_011515.12
Ensembl:   ENSG00000160310
Entrez:   3275
Chromosome Mapping:   21q22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997500
y-intercept 35.080000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download