PrimePCR™ SYBR® Green Assay: FYN, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0027682


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PrimePCR™ PreAmp for SYBR® Green Assay: FYN, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: FYN, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0010664
Assay Design:		Intron-spanning
Chromosome Location:		6:112035583-112041070question
Amplicon Length:		97
Splice Variants Targeted:		ENST00000368682 ENST00000354650 ENST00000229471 ENST00000368667 ENST00000368678 ENST00000462856 ENST00000520518 ENST00000517419 ENST00000518295 ENST00000523238 ENST00000524310 ENST00000523574 ENST00000462598 ENST00000518630 ENST00000523570 ENST00000484067 ENST00000521062 ENST00000229470 ENST00000356013 ENST00000538466

Gene Information

This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist. [provided by RefSeq Jul 2008]

Gene Symbol:		FYN
Gene Name:		FYN oncogene related to SRC, FGR, YES
Aliases:		MGC45350, SLK, SYN
RefSeq:		NC_000006.11 NT_025741.15
Ensembl:		ENSG00000010810
Entrez:		2534
Chromosome Mapping:		6q21

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999600
y-intercept	33.580000
Efficiency	101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.