PrimePCR™ SYBR® Green Assay: ENG, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0027737


List Price:		$179.00
Your Price:		Log In
Quantity:		Add to Cart Add to Cart
Add to My PrimePCR Add to My PrimePCR		Add to Quote Add to Quote
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: ENG, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

List Price:		$174.00
Your Price:		Log In
Quantity:		Add to Cart Add to Cart
[ Add to My PrimePCR ] [ Add to My PrimePCR ]		[ Add to Quote ] [ Add to Quote ]

PrimePCR™ Template for SYBR® Green Assay: ENG, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

List Price:		$179.00
Your Price:		Log In
Quantity:		Add to Cart Add to Cart
[ Add to My PrimePCR ] [ Add to My PrimePCR ]		[ Add to Quote ] [ Add to Quote ]

Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0010800
Assay Design:		Intron-spanning
Chromosome Location:		9:130581010-130582191question
Amplicon Length:		124
Splice Variants Targeted:		ENST00000373203 ENST00000344849 ENST00000545345 ENST00000546301

Gene Information

This gene encodes a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds TGFB1 and TGFB3 with high affinity. Mutations in this gene cause hereditary hemorrhagic telangiectasia also known as Osler-Rendu-Weber syndrome 1 an autosomal dominant multisystemic vascular dysplasia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Sep 2008]

Gene Symbol:		ENG
Gene Name:		endoglin
Aliases:		CD105, END, FLJ41744, HHT1, ORW, ORW1
RefSeq:		NC_000009.11 NG_009551.1 NG_023245.1 NT_008470.19
Ensembl:		ENSG00000106991
Entrez:		2022
Chromosome Mapping:		9q33-q34.1

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999800
y-intercept	35.470000
Efficiency	100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.