PrimePCR™ SYBR® Green Assay: IKZF1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: IKZF1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: IKZF1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010923
Assay Design:   Intron-spanning
Chromosome Location:   7:50358647-50367294question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000346667 ENST00000343574 ENST00000438033 ENST00000359197 ENST00000413698 ENST00000440768 ENST00000349824 ENST00000357364 ENST00000439701 ENST00000331340

Gene Information

This gene encodes a transcription factor that belongs to the family of zinc-finger DNA binding proteins associated with chromatin remodeling. The expression of this protein is restricted to the fetal and adult hemo-lymphopoietic system and it functions as a regulator of lymphocyte differentiation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. All isoforms share a common C-terminal domain which contains two zinc finger motifs that are required for hetero- or homodimerization and for interactions with other proteins. The isoforms however differ in the number of N-terminal zinc finger motifs that bind DNA and contain the nuclear localization signal resulting in members with and without DNA-binding properties. Only few isoforms contain the requisite three or more N-terminal zinc motifs that confer high affinity binding to a specific core DNA sequence element in the promoters of target genes. The non-DNA-binding isoforms are largely found in the cytoplasm and thought to function as dominant negative factors. Overexpression of some dominant-negative isoforms have been associated with B-cell malignancies such as acute lymphoblastic leukemia (ALL). [provided by RefSeq May 2011]

Gene Symbol:   IKZF1
Gene Name:   IKAROS family zinc finger 1 (Ikaros)
Aliases:   Hs.54452, IK1, IKAROS, LYF1, PRO0758, ZNFN1A1, hIk-1
RefSeq:   NC_000007.13 NT_007819.17 NT_033968.6
Ensembl:   ENSG00000185811
Entrez:   10320
Chromosome Mapping:   7p13-p11.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 33.750000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download