PrimePCR™ SYBR® Green Assay: TPD52L1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0027793


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PrimePCR™ PreAmp for SYBR® Green Assay: TPD52L1, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: TPD52L1, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0010936
Assay Design:		Intron-spanning
Chromosome Location:		6:125541266-125550365question
Amplicon Length:		146
Splice Variants Targeted:		ENST00000534368 ENST00000304877 ENST00000534000 ENST00000368402 ENST00000368388 ENST00000527711 ENST00000528193 ENST00000532429 ENST00000534199 ENST00000392482 ENST00000524679 ENST00000392484

Gene Information

This gene encodes a member of the tumor protein D52 (TPD52) family. The encoded protein contains a coiled-coil domain and may form homo- or hetero-dimer with TPD52 family members. The protein is reported to be involved in cell proliferation and calcium signaling. It also interacts with the mitogen-activated protein kinase kinase kinase 5 (MAP3K5/ASK1) and positively regulates MAP3K5-induced apoptosis. Multiple alternatively spliced transcript variants have been observed but the full-length nature of some variants has not yet been determined. [provided by RefSeq Jul 2008]

Gene Symbol:		TPD52L1
Gene Name:		tumor protein D52-like 1
Aliases:		D53, MGC8556, TPD52L2, hD53
RefSeq:		NC_000006.11 NT_025741.15
Ensembl:		ENSG00000111907
Entrez:		7164
Chromosome Mapping:		6q22-q23

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999700
y-intercept	35.170000
Efficiency	100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.