PrimePCR™ SYBR® Green Assay: CORO1C, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027872

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CORO1C, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CORO1C, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011120
Assay Design:   Intron-spanning
Chromosome Location:   12:109055824-109072091question
Amplicon Length:   125
Splice Variants Targeted:   ENST00000261401 ENST00000541050 ENST00000421578 ENST00000549772 ENST00000552871 ENST00000547294 ENST00000550032 ENST00000551550 ENST00000546571 ENST00000551044 ENST00000420959

Gene Information

This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD) which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes including cell cycle progression signal transduction apoptosis and gene regulation. [provided by RefSeq Jul 2008]

Gene Symbol:   CORO1C
Gene Name:   coronin, actin binding protein, 1C
Aliases:   HCRNN4
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000110880
Entrez:   23603
Chromosome Mapping:   12q24.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 34.940000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download