PrimePCR™ SYBR® Green Assay: EDNRB, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0028156


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PrimePCR™ PreAmp for SYBR® Green Assay: EDNRB, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: EDNRB, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0011733
Assay Design:		Intron-spanning
Chromosome Location:		13:78474054-78474773question
Amplicon Length:		137
Splice Variants Targeted:		ENST00000377211 ENST00000334286 ENST00000446573

Gene Information

The protein encoded by this gene is a G protein-coupled receptor which activates a phosphatidylinositol-calcium second messenger system. Its ligand endothelin consists of a family of three potent vasoactive peptides: ET1 ET2 and ET3. Studies suggest that the multigenic disorder Hirschsprung disease type 2 is due to mutations in the endothelin receptor type B gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jan 2011]

Gene Symbol:		EDNRB
Gene Name:		endothelin receptor type B
Aliases:		ABCDS, ETB, ETBR, ETRB, HSCR, HSCR2, WS4A
RefSeq:		NC_000013.10 NG_011630.2 NT_024524.14
Ensembl:		ENSG00000136160
Entrez:		1910
Chromosome Mapping:		13q22

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999000
y-intercept	35.610000
Efficiency	96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.