PrimePCR™ SYBR® Green Assay: SNX10, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0028158


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PrimePCR™ PreAmp for SYBR® Green Assay: SNX10, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: SNX10, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0011736
Assay Design:		Intron-spanning
Chromosome Location:		7:26404670-26411512question
Amplicon Length:		138
Splice Variants Targeted:		ENST00000416246 ENST00000338523 ENST00000396376 ENST00000409367 ENST00000409838 ENST00000446848

Gene Information

This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain which is a phosphoinositide binding domain and are involved in intracellular trafficking. This protein does not contain a coiled coil region like some family members. This gene may play a role in regulating endosome homeostasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq Dec 2010]

Gene Symbol:		SNX10
Gene Name:		sorting nexin 10
Aliases:		MGC33054
RefSeq:		NC_000007.13 NT_007819.17
Ensembl:		ENSG00000086300
Entrez:		29887
Chromosome Mapping:		7p15.2

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999100
y-intercept	35.130000
Efficiency	98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.