PrimePCR™ SYBR® Green Assay: EXTL1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: EXTL1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: EXTL1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012020
Assay Design:   Intron-spanning
Chromosome Location:   1:26358887-26359788question
Amplicon Length:   129
Splice Variants Targeted:   ENST00000374280

Gene Information

This gene is a member of the multiple exostoses (EXT) family of glycosyltransferases which function in the chain polymerization of heparan sulfate and heparin. The encoded protein harbors alpha 14- N-acetylglucosaminyltransferase activity and is involved in chain elongation of heparan sulfate and possibly heparin. [provided by RefSeq Jul 2008]

Gene Symbol:   EXTL1
Gene Name:   exostoses (multiple)-like 1
Aliases:   EXTL, MGC70794
RefSeq:   NC_000001.10 NT_004610.19
Ensembl:   ENSG00000158008
Entrez:   2134
Chromosome Mapping:   1p36.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996700
y-intercept 35.810000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download