PrimePCR™ SYBR® Green Assay: NMNAT2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0028299


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PrimePCR™ PreAmp for SYBR® Green Assay: NMNAT2, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: NMNAT2, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0012092
Assay Design:		Intron-spanning
Chromosome Location:		1:183247720-183253853question
Amplicon Length:		68
Splice Variants Targeted:		ENST00000287713 ENST00000294868

Gene Information

This gene product belongs to the nicotinamide mononucleotide adenylyltransferase (NMNAT) enzyme family members of which catalyze an essential step in NAD (NADP) biosynthetic pathway. Unlike the other human family member which is localized to the nucleus and is ubiquitously expressed; this enzyme is cytoplasmic and is predominantly expressed in the brain. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:		NMNAT2
Gene Name:		nicotinamide nucleotide adenylyltransferase 2
Aliases:		C1orf15, KIAA0479, MGC2756, PNAT2
RefSeq:		NC_000001.10 NT_004487.19
Ensembl:		ENSG00000157064
Entrez:		23057
Chromosome Mapping:		1q25

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998100
y-intercept	33.940000
Efficiency	100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.