PrimePCR™ SYBR® Green Assay: COL13A1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: COL13A1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: COL13A1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012225
Assay Design:   Intron-spanning
Chromosome Location:   10:71684719-71685622question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000398978 ENST00000354547 ENST00000357811 ENST00000520267 ENST00000517713 ENST00000522165 ENST00000520133 ENST00000398974 ENST00000398971 ENST00000398968 ENST00000398966 ENST00000398964 ENST00000398969 ENST00000356340 ENST00000398972 ENST00000398973

Gene Information

This gene encodes the alpha chain of one of the nonfibrillar collagens. The function of this gene product is not known however it has been detected at low levels in all connective tissue-producing cells so it may serve a general function in connective tissues. Unlike most of the collagens which are secreted into the extracellular matrix collagen XIII contains a transmembrane domain and the protein has been localized to the plasma membrane. The transcripts for this gene undergo complex and extensive splicing involving at least eight exons. Like other collagens collagen XIII is a trimer; it is not known whether this trimer is composed of one or more than one alpha chain isomer. A number of alternatively spliced transcript variants have been described but the full length nature of some of them has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   COL13A1
Gene Name:   collagen, type XIII, alpha 1
Aliases:   COLXIIIA1, FLJ42485
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000197467
Entrez:   1305
Chromosome Mapping:   10q22

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