PrimePCR™ SYBR® Green Assay: SCAP, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0028364


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PrimePCR™ PreAmp for SYBR® Green Assay: SCAP, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: SCAP, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0012264
Assay Design:		Intron-spanning
Chromosome Location:		3:47462453-47463985question
Amplicon Length:		91
Splice Variants Targeted:		ENST00000265565 ENST00000339815 ENST00000360832 ENST00000441517 ENST00000545718 ENST00000383739

Gene Information

This gene encodes a protein with a sterol sensing domain (SSD) and seven WD domains. In the presence of cholesterol this protein binds to sterol regulatory element binding proteins (SREBPs) and mediates their transport from the ER to the Golgi. The SREBPs are then proteolytically cleaved and regulate sterol biosynthesis. [provided by RefSeq Jul 2008]

Gene Symbol:		SCAP
Gene Name:		SREBF chaperone
Aliases:		KIAA0199
RefSeq:		NC_000003.11 NT_022517.18
Ensembl:		ENSG00000114650
Entrez:		22937
Chromosome Mapping:		3p21.31

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	1.000000
y-intercept	35.460000
Efficiency	101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.