PrimePCR™ SYBR® Green Assay: FN1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best coverage


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PrimePCR™ PreAmp for SYBR® Green Assay: FN1, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: FN1, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0012349
Assay Design:		Intron-spanning
Chromosome Location:		2:216288973-216289908question
Amplicon Length:		138
Splice Variants Targeted:		ENST00000421182 ENST00000323926 ENST00000336916 ENST00000357867 ENST00000354785 ENST00000446046 ENST00000443816 ENST00000432072 ENST00000356005 ENST00000426059 ENST00000265313 ENST00000359671 ENST00000346544 ENST00000345488 ENST00000357009

Gene Information

This gene encodes fibronectin a glycoprotein present in a soluble dimeric form in plasma and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis wound healing blood coagulation host defense and metastasis. The gene has three regions subject to alternative splicing with the potential to produce 20 different transcript variants. However the full-length nature of some variants has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:		FN1
Gene Name:		fibronectin 1
Aliases:		CIG, DKFZp686F10164, DKFZp686H0342, DKFZp686I1370, DKFZp686O13149, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF
RefSeq:		NC_000002.11 NT_005403.17 NG_012196.1
Ensembl:		ENSG00000115414
Entrez:		2335
Chromosome Mapping:		2q34

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999500
y-intercept	35.770000
Efficiency	94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.