PrimePCR™ SYBR® Green Assay: CDK5RAP1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028422

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CDK5RAP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CDK5RAP1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012381
Assay Design:   Intron-spanning
Chromosome Location:   20:31958341-31960503question
Amplicon Length:   121
Splice Variants Targeted:   ENST00000427097 ENST00000346416 ENST00000357886 ENST00000339269 ENST00000452723 ENST00000375351

Gene Information

Neuronal CDC2-like kinase which is involved in the regulation of neuronal differentiation is composed of a catalytic subunit CDK5 and an activating subunit p25NCK5A. The protein encoded by this gene binds to p25NCK5A and therefore may be involved in neuronal differentiation. Multiple transcript variants exist for this gene but the full-length natures of only two have been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   CDK5RAP1
Gene Name:   CDK5 regulatory subunit associated protein 1
Aliases:   C20orf34, C42, CDK5RAP1.3, CDK5RAP1.4, CGI-05, HSPC167
RefSeq:   NC_000020.10 NT_011362.10
Ensembl:   ENSG00000101391
Entrez:   51654
Chromosome Mapping:   20pter-q11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 34.790000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download