PrimePCR™ SYBR® Green Assay: TNFAIP2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028563

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TNFAIP2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TNFAIP2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012713
Assay Design:   Intron-spanning
Chromosome Location:   14:103600033-103601624question
Amplicon Length:   147
Splice Variants Targeted:   ENST00000333007 ENST00000451723 ENST00000538222

Gene Information

This gene was identified as a gene whose expression can be induced by the tumor necrosis factor alpha (TNF) in umbilical vein endothelial cells. The expression of this gene was shown to be induced by retinoic acid in a cell line expressing a oncogenic version of the retinoic acid receptor alpha fusion protein which suggested that this gene may be a retinoic acid target gene in acute promyelocytic leukemia. [provided by RefSeq Jul 2008]

Gene Symbol:   TNFAIP2
Gene Name:   tumor necrosis factor, alpha-induced protein 2
Aliases:   B94, EXOC3L3
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000185215
Entrez:   7127
Chromosome Mapping:   14q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997300
y-intercept 35.220000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download