PrimePCR™ SYBR® Green Assay: CLCA4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028689

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CLCA4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CLCA4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013017
Assay Design:   Intron-spanning
Chromosome Location:   1:87033233-87036805question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000263723 ENST00000370563

Gene Information

The protein encoded by this gene belongs to the calcium sensitive chloride conductance protein family. To date all members of this gene family map to the same site on chromosome 1p31-p22 and share high degrees of homology in size sequence and predicted structure but differ significantly in their tissue distributions. Alternative splicing results in multiple transcript variants only one of which is thought to be protein coding. [provided by RefSeq Dec 2008]

Gene Symbol:   CLCA4
Gene Name:   chloride channel accessory 4
Aliases:   CaCC, CaCC2, MGC142247, MGC142249
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000016602
Entrez:   22802
Chromosome Mapping:   1p31-p22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.170000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download