PrimePCR™ SYBR® Green Assay: ANKMY1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: ANKMY1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ANKMY1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013312
Assay Design:   Intron-spanning
Chromosome Location:   2:241463708-241465157question
Amplicon Length:   117
Splice Variants Targeted:   ENST00000373318 ENST00000272972 ENST00000361678 ENST00000403283 ENST00000401804 ENST00000405523 ENST00000405002 ENST00000391987 ENST00000373320 ENST00000536462 ENST00000539830

Gene Information

Description Not Available

Gene Symbol:   ANKMY1
Gene Name:   ankyrin repeat and MYND domain containing 1
Aliases:   DKFZp686D20155, DKFZp686L21237, FLJ20499, ZMYND13
RefSeq:   NC_000002.11 NT_005416.13
Ensembl:   ENSG00000144504
Entrez:   51281
Chromosome Mapping:   2q37.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.950000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download