PrimePCR™ SYBR® Green Assay: SEMA3F, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0029001

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SEMA3F, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SEMA3F, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013705
Assay Design:   Intron-spanning
Chromosome Location:   3:50214237-50219766question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000414301 ENST00000450338 ENST00000413852 ENST00000002829 ENST00000434342

Gene Information

The semaphorins are a family of proteins that are involved in signaling. All the family members have a secretion signal a 500-amino acid sema domain and 16 conserved cysteine residues (Kolodkin et al. 1993 [PubMed 8269517]). Sequence comparisons have grouped the secreted semaphorins into 3 general classes all of which also have an immunoglobulin domain. The semaphorin III family consisting of human semaphorin III (SEMA3A; MIM 603961) chicken collapsin and mouse semaphorins A D and E all have a basic domain at the C terminus. Chicken collapsin contributes to path finding by axons during development by inhibiting extension of growth cones (Luo et al. 1993 [PubMed 8402908]) through an interaction with a collapsin response mediator protein of relative molecular mass 62K (CRMP62) (Goshima et al. 1995 [PubMed 7637782]) a putative homolog of an axonal guidance associated UNC33 gene product (MIM 601168). SEMA3F is a secreted member of the semaphorin III family.[supplied by OMIM Mar 2008]

Gene Symbol:   SEMA3F
Gene Name:   sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F
Aliases:   SEMA-IV, SEMA4, SEMAK
RefSeq:   NC_000003.11 NG_009831.1 NT_022517.18
Ensembl:   ENSG00000001617
Entrez:   6405
Chromosome Mapping:   3p21.3

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