PrimePCR™ SYBR® Green Assay: TBL1XR1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best coverage


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PrimePCR™ PreAmp for SYBR® Green Assay: TBL1XR1, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: TBL1XR1, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0013861
Assay Design:		Intron-spanning
Chromosome Location:		3:176771565-176782780question
Amplicon Length:		185
Splice Variants Targeted:		ENST00000430069 ENST00000457928 ENST00000352800 ENST00000437738 ENST00000450267 ENST00000431674 ENST00000422066 ENST00000443315 ENST00000422442

Gene Information

The protein encoded by this gene has sequence similarity with members of the WD40 repeat-containing protein family. The WD40 group is a large family of proteins which appear to have a regulatory function. It is believed that the WD40 repeats mediate protein-protein interactions and members of the family are involved in signal transduction RNA processing gene regulation vesicular trafficking cytoskeletal assembly and may play a role in the control of cytotypic differentiation. [provided by RefSeq Jul 2008]

Gene Symbol:		TBL1XR1
Gene Name:		transducin (beta)-like 1 X-linked receptor 1
Aliases:		C21, DC42, FLJ12894, IRA1, TBLR1
RefSeq:		NC_000003.11 NT_005612.16
Ensembl:		ENSG00000177565
Entrez:		79718
Chromosome Mapping:		3q26.32

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.997700
y-intercept	35.130000
Efficiency	101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.