PrimePCR™ SYBR® Green Assay: TREM2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0029112


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PrimePCR™ PreAmp for SYBR® Green Assay: TREM2, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: TREM2, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0013940
Assay Design:		Intron-spanning
Chromosome Location:		6:41129249-41130878question
Amplicon Length:		171
Splice Variants Targeted:		ENST00000373113 ENST00000338469 ENST00000373122

Gene Information

The protein encoded by this gene is a membrane protein that forms a receptor signaling complex with TYROBP. The encoded protein may be involved in chronic inflammation by triggering the production of constitutive inflammatory cytokines. Defects in this gene are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL). [provided by RefSeq Jul 2008]

Gene Symbol:		TREM2
Gene Name:		triggering receptor expressed on myeloid cells 2
Aliases:		TREM-2, Trem2a, Trem2b, Trem2c
RefSeq:		NC_000006.11 NG_011561.1 NT_007592.15
Ensembl:		ENSG00000095970
Entrez:		54209
Chromosome Mapping:		6p21.1

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998500
y-intercept	35.820000
Efficiency	99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.