PrimePCR™ SYBR® Green Assay: PPP1R3C, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best coverage


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PrimePCR™ PreAmp for SYBR® Green Assay: PPP1R3C, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: PPP1R3C, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0014005
Assay Design:		Intron-spanning
Chromosome Location:		10:93390516-93392806question
Amplicon Length:		172
Splice Variants Targeted:		ENST00000238994 ENST00000500094

Gene Information

This gene encodes a regulatory subunit of protein phosphatase-1 (PP1). PP1 catalyzes reversible protein phosphorylation which is important in a wide range of cellular activities: neuronal muscular RNA splicing protein synthesis cell death and glycogen metabolism to name just a few. By interacting with different regulatory subunits PP1 is directed to different parts of the cell to different substrates or to respond to extracellular signals. [provided by RefSeq Oct 2011]

Gene Symbol:		PPP1R3C
Gene Name:		protein phosphatase 1, regulatory (inhibitor) subunit 3C
Aliases:		PPP1R5
RefSeq:		NC_000010.10 NT_030059.13
Ensembl:		ENSG00000119938
Entrez:		5507
Chromosome Mapping:		10q23-q24

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999500
y-intercept	35.320000
Efficiency	99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.