PrimePCR™ SYBR® Green Assay: HPN, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0029179


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PrimePCR™ PreAmp for SYBR® Green Assay: HPN, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: HPN, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0014067
Assay Design:		Intron-spanning
Chromosome Location:		19:35551634-35556172question
Amplicon Length:		77
Splice Variants Targeted:		ENST00000262626 ENST00000392226 ENST00000541345

Gene Information

This gene encodes a type II transmembrane serine protease. The encoded protein has an extracellular region that consists of two domains a catalytic serine protease domain and a non-catalytic scavenger receptor cysteine-rich domain. This protein may be involved in diverse cellular functions including blood coagulation maintenance of cell morphology and the growth and progression of certain cancers particularly prostate cancer. Alternative splicing results in multiple transcript variants.[provided by RefSeq Apr 2009]

Gene Symbol:		HPN
Gene Name:		hepsin
Aliases:		TMPRSS1
RefSeq:		NC_000019.9 NG_013359.1 NT_011109.16
Ensembl:		ENSG00000105707
Entrez:		3249
Chromosome Mapping:		19q11-q13.2

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998400
y-intercept	35.410000
Efficiency	100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.