PrimePCR™ SYBR® Green Assay: WT1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0029257

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: WT1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: WT1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0014203
Assay Design:   Intron-spanning
Chromosome Location:   11:32417814-32421550question
Amplicon Length:   167
Splice Variants Targeted:   ENST00000379079 ENST00000332351 ENST00000530998 ENST00000452863 ENST00000448076 ENST00000527882 ENST00000526685

Gene Information

This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system and it is mutated in a small subset of patients with Wilm's tumors. This gene exhibits complex tissue-specific and polymorphic imprinting pattern with biallelic and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants there is evidence for the use of a non-AUG (CUG) translation initiation site upstream of and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq Oct 2010]

Gene Symbol:   WT1
Gene Name:   Wilms tumor 1
Aliases:   AWT1, GUD, NPHS4, WAGR, WIT-2, WT33
RefSeq:   NC_000011.9 NT_009237.18 NG_009272.1
Ensembl:   ENSG00000184937
Entrez:   7490
Chromosome Mapping:   11p13

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