PrimePCR™ SYBR® Green Assay: SLC3A2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: SLC3A2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SLC3A2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0014525
Assay Design:   Intron-spanning
Chromosome Location:   11:62652978-62655817question
Amplicon Length:   171
Splice Variants Targeted:   ENST00000422606 ENST00000377890 ENST00000542007 ENST00000377889 ENST00000535296 ENST00000338663 ENST00000539891 ENST00000536981 ENST00000377892 ENST00000377891

Gene Information

This gene is a member of the solute carrier family and encodes a cell surface transmembrane protein. The protein exists as the heavy chain of a heterodimer covalently bound through di-sulfide bonds to one of several possible light chains. The encoded transporter plays a role in regulation of intracellular calcium levels and transports L-type amino acids. Alternatively spliced transcript variants encoding different isoforms have been characterized. [provided by RefSeq Nov 2010]

Gene Symbol:   SLC3A2
Gene Name:   solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2
Aliases:   4F2, 4F2HC, 4T2HC, CD98, CD98HC, MDU1, NACAE
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000168003
Entrez:   6520
Chromosome Mapping:   11q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 35.770000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download