PrimePCR™ SYBR® Green Assay: XRN1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

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PrimePCR™ PreAmp for SYBR® Green Assay: XRN1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: XRN1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0014681
Assay Design:   Intron-spanning
Chromosome Location:   3:142141677-142144065question
Amplicon Length:   142
Splice Variants Targeted:   ENST00000264951 ENST00000392981 ENST00000463916 ENST00000477237 ENST00000544157

Gene Information

SEP1 localizes to cytoplasmic foci containing a complex of mRNA-degrading enzymes. In addition to mRNA metabolism yeast Sep1 has been implicated in a variety of nuclear and cytoplasmic functions including homologous recombination meiosis telomere maintenance and microtubule assembly.[supplied by OMIM Apr 2004]

Gene Symbol:   XRN1
Gene Name:   5'-3' exoribonuclease 1
Aliases:   DKFZp434P0721, DKFZp686B22225, DKFZp686F19113, FLJ41903, SEP1
RefSeq:   NG_008951.1 NC_000003.11 NT_005612.16
Ensembl:   ENSG00000114127
Entrez:   54464
Chromosome Mapping:   3q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.560000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download