PrimePCR™ SYBR® Green Assay: SKIL, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0029527

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SKIL, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SKIL, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0014721
Assay Design:   Intron-spanning
Chromosome Location:   3:170108943-170110117question
Amplicon Length:   147
Splice Variants Targeted:   ENST00000259119 ENST00000426052 ENST00000413427 ENST00000458537

Gene Information

The protein encoded by this gene is a component of the SMAD pathway which regulates cell growth and differentiation through transforming growth factor-beta (TGFB). In the absence of ligand the encoded protein binds to the promoter region of TGFB-responsive genes and recruits a nuclear repressor complex. TGFB signaling causes SMAD3 to enter the nucleus and degrade this protein allowing these genes to be activated. Four transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq Oct 2011]

Gene Symbol:   SKIL
Gene Name:   SKI-like oncogene
Aliases:   SNO, SnoA, SnoI, SnoN
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000136603
Entrez:   6498
Chromosome Mapping:   3q26

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997800
y-intercept 36.610000
Efficiency 89

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download