PrimePCR™ SYBR® Green Assay: CNRIP1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0029796


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PrimePCR™ PreAmp for SYBR® Green Assay: CNRIP1, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: CNRIP1, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0015231
Assay Design:		Intron-spanning
Chromosome Location:		2:68544354-68546428question
Amplicon Length:		131
Splice Variants Targeted:		ENST00000409559 ENST00000263655 ENST00000409862

Gene Information

This gene encodes a G-protein coupled receptor which interacts with the C-terminal tail of cannabinoid receptor 1. This receptor plays a role in synaptic plasticity analgesia appetite and neuroprotection. Two transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:		CNRIP1
Gene Name:		cannabinoid receptor interacting protein 1
Aliases:		C2orf32, CRIP1, DKFZp566K1924
RefSeq:		NC_000002.11 NT_022184.15
Ensembl:		ENSG00000119865
Entrez:		25927
Chromosome Mapping:		2p14

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999300
y-intercept	35.430000
Efficiency	94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.