PrimePCR™ SYBR® Green Assay: RCN2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0029975


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PrimePCR™ PreAmp for SYBR® Green Assay: RCN2, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: RCN2, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0015492
Assay Design:		Intron-spanning
Chromosome Location:		15:77236165-77240756question
Amplicon Length:		120
Splice Variants Targeted:		ENST00000394885 ENST00000320963 ENST00000394883

Gene Information

The protein encoded by this gene is a calcium-binding protein located in the lumen of the ER. The protein contains six conserved regions with similarity to a high affinity Ca(+2)-binding motif the EF-hand. This gene maps to the same region as type 4 Bardet-Biedl syndrome suggesting a possible causative role for this gene in the disorder. [provided by RefSeq Feb 2009]

Gene Symbol:		RCN2
Gene Name:		reticulocalbin 2, EF-hand calcium binding domain
Aliases:		E6BP, ERC-55, ERC55, TCBP49
RefSeq:		NC_000015.9 NT_010194.17
Ensembl:		ENSG00000117906
Entrez:		5955
Chromosome Mapping:		15q23

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999200
y-intercept	34.540000
Efficiency	99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.