PrimePCR™ SYBR® Green Assay: SLC5A8, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0029977


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PrimePCR™ PreAmp for SYBR® Green Assay: SLC5A8, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: SLC5A8, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0015496
Assay Design:		Intron-spanning
Chromosome Location:		12:101598296-101603390question
Amplicon Length:		133
Splice Variants Targeted:		ENST00000536262

Gene Information

SLC5A8 has been shown to transport iodide by a passive mechanism (Rodriguez et al. 2002 [PubMed 12107270]) and monocarboxylates and short-chain fatty acids by a sodium-coupled mechanism (Gopal et al. 2004 [PubMed 15322102]). In kidney SLC5A8 functions as a high-affinity sodium-coupled lactate transporter involved in reabsorption of lactate and maintenance of blood lactate levels (Thangaraju et al. 2006 [PubMed 16873376]).[supplied by OMIM Dec 2008]

Gene Symbol:		SLC5A8
Gene Name:		solute carrier family 5 (iodide transporter), member 8
Aliases:		AIT, MGC125354, SMCT, SMCT1
RefSeq:		NC_000012.11 NT_029419.12 NW_003315942.1
Ensembl:		ENSG00000256870
Entrez:		160728
Chromosome Mapping:		12q23.1

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998600
y-intercept	34.410000
Efficiency	98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.