PrimePCR™ SYBR® Green Assay: GNGT2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0029999


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PrimePCR™ PreAmp for SYBR® Green Assay: GNGT2, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: GNGT2, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0015528
Assay Design:		Intron-spanning
Chromosome Location:		17:47284722-47286242question
Amplicon Length:		91
Splice Variants Targeted:		ENST00000503070 ENST00000511277 ENST00000300406 ENST00000515635 ENST00000507680 ENST00000511673

Gene Information

Phototransduction in rod and cone photoreceptors is regulated by groups of signaling proteins. The encoded protein is thought to play a crucial role in cone phototransduction. It belongs to the G protein gamma family and localized specifically in cones. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq Nov 2010]

Gene Symbol:		GNGT2
Gene Name:		guanine nucleotide binding protein (G protein), gamma transducing activity polypeptide 2
Aliases:		G-GAMMA-8, G-GAMMA-C, GNG8, GNG9, GNGT8
RefSeq:		NC_000017.10 NG_009841.1 NT_010783.15
Ensembl:		ENSG00000167083
Entrez:		2793
Chromosome Mapping:		17q21

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999200
y-intercept	34.390000
Efficiency	99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.