PrimePCR™ SYBR® Green Assay: RASA3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0030094

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RASA3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RASA3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0015684
Assay Design:   Intron-spanning
Chromosome Location:   13:114786891-114789811question
Amplicon Length:   121
Splice Variants Targeted:   ENST00000334062 ENST00000389544 ENST00000542651

Gene Information

The protein encoded by this gene is member of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS thereby allowing control of cellular proliferation and differentiation. This family member is an inositol 1345-tetrakisphosphate-binding protein like the closely related RAS p21 protein activator 2. The two family members have distinct pleckstrin-homology domains with this particular member having a domain consistent with its localization to the plasma membrane. [provided by RefSeq Jul 2008]

Gene Symbol:   RASA3
Gene Name:   RAS p21 protein activator 3
Aliases:   GAP1IP4BP, GAPIII, MGC46517, MGC47588
RefSeq:   NC_000013.10 NT_024498.12
Ensembl:   ENSG00000185989
Entrez:   22821
Chromosome Mapping:   13q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.670000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download