PrimePCR™ SYBR® Green Assay: DDX5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0030531

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: DDX5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DDX5, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0016345
Assay Design:   Intron-spanning
Chromosome Location:   17:62496825-62498186question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000540698 ENST00000450599 ENST00000225792

Gene Information

DEAD box proteins characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD) are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation nuclear and mitochondrial splicing and ribosome and spliceosome assembly. Based on their distribution patterns some members of this family are believed to be involved in embryogenesis spermatogenesis and cellular growth and division. This gene encodes a DEAD box protein which is a RNA-dependent ATPase and also a proliferation-associated nuclear antigen specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons and alternatively spliced transcripts containing several intron sequences have been detected but no isoforms encoded by these transcripts have been identified. [provided by RefSeq Jul 2008]

Gene Symbol:   DDX5
Gene Name:   DEAD (Asp-Glu-Ala-Asp) box polypeptide 5
Aliases:   DKFZp434E109, DKFZp686J01190, G17P1, HLR1, HUMP68, p68
RefSeq:   NC_000017.10 NG_013029.1 NT_010783.15 NW_003315947.1
Ensembl:   ENSG00000108654
Entrez:   1655
Chromosome Mapping:   17q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 34.750000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download