PrimePCR™ SYBR® Green Assay: ORC1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0030768

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ORC1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ORC1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0016725
Assay Design:   Intron-spanning
Chromosome Location:   1:52847339-52849154question
Amplicon Length:   128
Splice Variants Targeted:   ENST00000371568 ENST00000371566

Gene Information

The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is the largest subunit of the ORC complex. While other ORC subunits are stable throughout the cell cycle the levels of this protein vary during the cell cycle which has been shown to be controlled by ubiquitin-mediated proteolysis after initiation of DNA replication. This protein is found to be selectively phosphorylated during mitosis. It is also reported to interact with MYST histone acetyltransferase 2 (MyST2/HBO1) a protein involved in control of transcription silencing. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jun 2010]

Gene Symbol:   ORC1
Gene Name:   origin recognition complex, subunit 1
Aliases:   HSORC1, ORC1L, PARC1
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000085840
Entrez:   4998
Chromosome Mapping:   1p32

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